Biomedical Application

Non-Invasive Quantitative Acoustic Imaging of Intact Brain Organoids for Structural Assessment in Drug Discovery

Human brain organoids were imaged using high-frequency Scanning Acoustic Microscopy (SAM) with 40 MHz and 60 MHz transducers to quantitatively assess tissue heterogeneity. Statistical analysis of acoustic scattering using Rayleigh and Nakagami models showed that the Nakagami distribution better captured microstructural variations across different organoid regions compared with conventional B-mode imaging. The results demonstrate that statistical acoustic imaging provides a non-invasive and quantitative approach for characterizing brain organoid architecture and developmental heterogeneity.